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In addition, several previous studies have suggested that EMT was associated with bladder cancer progression and metastasis (Chaffer et al, 2006; Mc Conkey et al, 2009; Kenney et al, 2011; Wiklund et al, 2011).Both Cox-2 overexpression and the loss of E-cadherin expression are frequently detected in invasive bladder cancer (Shariat et al, 2001).The extracted m RNA was reverse transcribed using the Taq-Man Reverse Transcription Reagents kit (Applied Biosystems, Foster City, CA, USA).Real-time quantitative PCR using the SYBR Green I dye fluoresces system (Applied Biosystems) was performed for the relative quantification of the m RNA expression according to a previously described method (Guo et al, 2006).In this study, we investigated the effect of Cox-2 inhibitors on reversal EMT and tumour growth inhibition in bladder cancer cells.
They found that the expression of the mesenchymal markers was confined to the muscle-invasive tumour.Bladder cancer, transitional cell carcinoma (TCC) of the urinary bladder, accounts for approximately 4.6% of all malignant tumour (Jemal et al, 2007).
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“That car had a whole trunk full of computer equipment,” Audi spokesman Brad Stertz said of the self-parking car that Audi debuted in 2013. Several million people that had fled the Judeo - Communist death machine to western Europe , by agreement , were sent back to the Soviet Union after the war . Consistent with that experience and the record built in this proceeding, today we adopt carefully-tailored rules that would prevent specific practices we know are harmful to Internet openness— blocking, throttling, and paid prioritization—as well as a strong standard of conduct designed to prevent the deployment of new practices that would harm Internet openness.… continue reading »